WisdomInterface

The CTS Rotea system: automation and consistency in PBMC separation—a comparison of protocols

There are several manual methods to separate peripheral blood mononuclear cells (PBMCs) from leukapheresis products, but most of them are highly user‑dependent and laborious. The Gibco™ CTS™ Rotea™ Counterflow Centrifugation System can be used to automate a variety of methods to separate PBMCs in a closed environment.

In this application note, you’ll see effective and consistent PBMC separation from the CTS Rotea system across all tested methods compared to a manual density gradient centrifugation method.

Test methods include:

  • Ficoll™ solution
  • With ACK (ammonium-chloride-potassium) lysis buffer
  • Without lysis buffer
SUBSCRIBE
Image Processing

Who We Are

Xcellent Insights is a futuristic market intelligence firm, headquartered in New York, US that focuses on providing strategic market insights. We offer a plethora of data-centric research reports and consulting services to help customers expand and explore their business strategies, obtain clarity about current business trends and future development, and attain substantial growth. Our portfolio of services includes syndicated and custom research reports driven by end-to-end market research and market intelligence studies.

What We Do

We strive to offer the best market research and consulting services to our customers that will benefit them in making informed business decisions. We provide an extensive list of market research titles falling under various industry categories such as consumer goods, food, and beverages, automotive, healthcare, and chemicals, covering latest and trending market insights. Through our services, we aim to connect an organization’s goal with lucrative outcomes globally.

    Subscribe for more insights



    By completing and submitting this form, you understand and agree to WisdomInterface processing your acquired contact information as described in our privacy policy.

    No spam, we promise. You can update your email preference or unsubscribe at any time and we'll never share your details without your permission.

      Subscribe for more insights



      By completing and submitting this form, you understand and agree to WisdomInterface processing your acquired contact information as described in our privacy policy.

      No spam, we promise. You can update your email preference or unsubscribe at any time and we'll never share your details without your permission.